This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (PDMS) microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope (CLSM). The microfluidic sensor along with 1-TDPPOin this study exhibits the following advantages: (i) Real-time chemo-repellent responses of cell dynamics; (ii) Rapid eradication of biofilm by embedded obstacles and powerful antibacterial agents, which significantly reduce the response time compared to classical methods; (iii) Minimal consumption of cells and antimicrobial agents; and (iv) Simplifying the process of the normalization of the fluorescence intensity and monitoring of biofilm by captured images and datasets.
Changhyun Roh 1,†, Jaewoong Lee 2,†, Mayank Kinger 3 and Chankyu Kang 4,*
1 Biotechnology Research Division, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 1266, Sinjeong-Dong, Jeongeup, Jeonbuk 580-185, Korea
2 Department of Textile Engineering and Technology, Yeungnam University, 280 Daehak-ro, Gyeongsan, Gyeongbuk 38541, Korea
3 Department of Chemistry, Maharishi Markandeshwar University, Mullana, (Ambala) Haryana 133207, India
4 Ministry of Employment and Labor, Major Industrial Accident Prevention Center, 34 Yeosusandallo, Yeosu-Si, Jeonnam 59631, Korea
† These authors contributed equally to this work.
* Author to whom correspondence should be addressed.