[Chemical Communications] Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli

March 31, 2017

 

Abstract:

 

We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs and microemboli by 1.6 and 3.0-fold, respectively (both P < 0.05).

 

Chian-Hui Lai,ab  Syer Choon Lim,a  Liang-Chun Wu,a  Chien-Fang Wang,a  Wen-Sy Tsai,cd  Han-Chung Wue  and  Ying-Chih Chang*af 

 Author affiliations

*Corresponding authors

aGenomics Research Center, Academia Sinica, Taipei, Taiwan 
E-mail: yingchih@gate.sinica.edu.tw

bDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan

cGraduate Institute of Clinical Medical Science, Chang-Gung University, Taoyuan, Taiwan

dDivision of Colon and Rectal Surgery, Chang-Gung Memorial Hospital, Taoyuan, Taiwan

eInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan

fDepartment of Chemical Engineering, Stanford University, Stanford, USA

 

Link: http://pubs.rsc.org/en/Content/ArticleLanding/2017/CC/C7CC00247E#!divAbstract

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