We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs and microemboli by 1.6 and 3.0-fold, respectively (both P < 0.05).
Chian-Hui Lai,ab Syer Choon Lim,a Liang-Chun Wu,a Chien-Fang Wang,a Wen-Sy Tsai,cd Han-Chung Wue and Ying-Chih Chang*af
aGenomics Research Center, Academia Sinica, Taipei, Taiwan
bDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan
cGraduate Institute of Clinical Medical Science, Chang-Gung University, Taoyuan, Taiwan
dDivision of Colon and Rectal Surgery, Chang-Gung Memorial Hospital, Taoyuan, Taiwan
eInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
fDepartment of Chemical Engineering, Stanford University, Stanford, USA