[Patent] Methods and devices for electrical sample preparation
Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
Publication number US20170211128 A1 Publication type Application Application number US 15/401,291 Publication date Jul 27, 2017 Filing date Jan 9, 2017 Priority date Jul 25, 2011 Also published as CA2842720A1, 5 More » Inventors Samad Talebpour, Aye Aye Khine, Robert Maaskant, Tino Alavie Original Assignee Qvella Corporation