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[Analytical Chemistry] Integrated smartphone-app-chip system for on-site ppb-level colorimetric quan


We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional ELISA kits were obtained. The achieved detection limit (3±1 μg/kg, equivalent to ppb) and dynamic response range (0.5 to 250 μg/kg) meet the requested testing standards set by authorities worldwide. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.

Xiaochun Li, Fan Yang, Jessica X. H. Wong, and Hua-Zhong Yu Anal. Chem., Just Accepted Manuscript DOI: 10.1021/acs.analchem.7b01379 Publication Date (Web): July 18, 2017 Copyright © 2017 American Chemical Society

Link: http://pubs.acs.org/doi/abs/10.1021/acs.analchem.7b01379

#07202017 #microchannels

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