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[Biomicrofluidics] Automated selection of aptamers against cholangiocarcinoma cells on an integrated


Abstract:

An integrated microfluidic system capable of automatically identifying aptamers specific to cholangiocarcinoma (CCA) cells was developed herein. The developed system was capable of performing cell-based systematic evolution of ligands via an exponential enrichment (Cell-SELEX) process on-chip, and only six rounds of Cell-SELEX were required to identify high specificity aptamers; this represents a significant improvement in speed over conventional SELEX, in which 15–20 rounds are typically required. Using the microfluidic chip developed, three aptamers specific to CCA cells (one for SNU-478 cells and two for HuCCT-1 cells) were successfully screened. This automated system could be modified to uncover aptamer probes against other cancer cells, thereby allowing for earlier diagnosis and consequently a potentially improved prognosis.

Priya Gopinathan1, Lien-Yu Hung2, Chih-Hung Wang2, Nai-Jung Chiang3,4, Yu-Chun Wang5, Yan-Shen Shan5,a), and Gwo-Bin Lee1,2,a)lessHide Affiliations 1Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan 2Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan 3National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan 4Division of Hematology and Oncology, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan 5Institute of Clinical Medicine, National Cheng Kung University Hospital, Tainan, Taiwan a)Authors to whom correspondence should be addressed: gwobin@pme.nthu.edu.tw, Tel.: +886-3-5715131 ext. 33765, Fax: +886-3-5742495 and ysshan@mail.ncku.edu.tw, Tel.: +886-6-2353535 ext. 3105, Fax: +886-6-2758781.

Note: The preliminary results of this study have been presented at the MicroTAS 2016, Dublin, 2016.

Biomicrofluidics 11, 044101 (2017); doi: http://dx.doi.org/10.1063/1.4991005

Link: http://aip.scitation.org/doi/abs/10.1063/1.4991005

#07192017 #labonachip #integratedonchip

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