[Sensors] Novel Selective Detection Method of Tumor Angiogenesis Factors Using Living Nano-Robots
This paper reports a novel self-detection method for tumor cells using living nano-robots. These living robots are a nonpathogenic strain of E. coli bacteria equipped with naturally synthesized bio-nano-sensory systems that have an affinity to VEGF, an angiogenic factor overly-expressed by cancer cells. The VEGF-affinity/chemotaxis was assessed using several assays including the capillary chemotaxis assay, chemotaxis assay on soft agar, and chemotaxis assay on solid agar. In addition, a microfluidic device was developed to possibly discover tumor cells through the overexpressed vascular endothelial growth factor (VEGF). Various experiments to study the sensing characteristic of the nano-robots presented a strong response toward the VEGF. Thus, a new paradigm of selective targeting therapies for cancer can be advanced using swimming E. coli as self-navigator miniaturized robots as well as drug-delivery vehicles.
Mohamed Al-Fandi 1,3,* OrcID, Nida Alshraiedeh 2, Rami Owies 1, Hala Alshdaifat 3, Omamah Al-Mahaseneh 3, Khadijah Al-Tall 3 and Rawan Alawneh 3 1 Nanotechnology Institute, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan 2 Faculty of Pharmacy, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan 3 Micro and Nano Systems Lab., Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan * Author to whom correspondence should be addressed. Received: 8 May 2017 / Revised: 24 June 2017 / Accepted: 1 July 2017 / Published: 14 July 2017