[ACS Appl. Mater. Interfaces] Amplified Micromagnetic Field Gradients Enable High-Resolution Profili
Analyzing small collections of cells is challenging because of the need for extremely high levels of sensitivity. We recently reported a new approach, termed magnetic ranking cytometry (MagRC), to profile nanoparticle-labeled cells. Using antibody-functionalized magnetic nanoparticles, we label cells so that each cell’s magnetization is proportional to its surface expression of a selected biomarker. Using a microfluidic device that sorts the cells into 100 different zones based on that magnetism, we generate profiles that report on the level and distribution of surface expression in small collections of cells. Here, we present a new set of studies investigating in depth parameters such as flow rate and magnetic nanoparticle size that affect device performance using both experiments and modeling. We present a model that further elucidates the mechanism of cell capture and use it model to optimize device performance in order to efficiently capture rare cells. We show that this method has excellent specificity and can be used to characterize rare cells even in the presence of whole blood.
Mahla Poudineh, Edward H. Sargent, and Shana O. Kelley ACS Appl. Mater. Interfaces, Just Accepted Manuscript DOI: 10.1021/acsami.7b04677 Publication Date (Web): July 11, 2017 Copyright © 2017 American Chemical Society