[Lab on a chip] Spectral reading of optical resonance-encoded cells in microfluidics
The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes. A microfluidic chip was used to measure fluorescence resonance peaks emitted from individual cells. An algorithm comparing the peak wavelengths to a reference barcode library allowed barcode identification with high accuracy. This work provides a guideline to increase the number of unique identifiers and reduce various false-positive and false-negative errors.
Matjaž Humar,abc Avinash Upadhyaad and Seok Hyun Yun*ae Author affiliations * Corresponding authors a Wellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, 65 Landsdowne St. UP-5, Cambridge, USA E-mail: firstname.lastname@example.org b Condensed Matter Department, J. Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia c Faculty of Mathematics and Physics, University of Ljubljana, Jadranska 19, Ljubljana, Slovenia d Research School of Engineering, Australian National University, North Road Canberra, Canberra, Australia e Harvard-MIT Health Sciences and Technology, 77 Massachusetts Avenue Cambridge, Cambridge, USA