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[ACS Nano] Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic


Abstract:

Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30–200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size-dependent, and label-free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.

Chao Liu‡, Jiayi Guo‡∥, Fei Tian‡, Na Yang‡, Fusheng Yan‡, Yanping Ding‡, JingYan Wei∥, Guoqing Hu*†§, Guangjun Nie*‡§ , and Jiashu Sun*‡§

† State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China

‡ CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China

§ University of Chinese Academy of Sciences, Beijing 100049, China

∥ College of Pharmaceutical Science, Jilin University, Changchun 130021, China

ACS Nano, Article ASAP

DOI: 10.1021/acsnano.7b02277

Publication Date (Web): July 5, 2017

Copyright © 2017 American Chemical Society

*E-mail: guoqing.hu@imech.ac.cn., *E-mail: niegj@nanoctr.cn., *E-mail: sunjs@nanoctr.cn.

Link: http://pubs.acs.org/doi/abs/10.1021/acsnano.7b02277

#07102017 #exosome #vesicle #microchannels

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