[Lab on a chip] Thermoplastic elastomer with advanced hydrophilization and bonding performances for
One of the most important areas of research on microfluidic technologies focuses on the identification and characterisation of novel materials with enhanced properties and versatility. Here we present a fast, easy and inexpensive microstructuration method for the fabrication of novel, flexible, transparent and biocompatible microfluidic devices. Using a simple hot press, we demonstrate the rapid (30 s) production of various microfluidic prototypes embossed in a commercially available soft thermoplastic elastomer (sTPE). This styrenic block copolymer (BCP) material is as flexible as PDMS and as thermoformable as classical thermoplastics. It exhibits high fidelity of replication using SU-8 and epoxy master molds in a highly convenient low-isobar (0.4 bar) and iso-thermal process. Microfluidic devices can then be easily sealed using either a simple hot plate or even a room-temperature assembly, allowing them to sustain liquid pressures of 2 and 0.6 bar, respectively. The excellent sorption and biocompatibility properties of the microchips were validated via a standard rhodamine dye assay as well as a sensitive yeast cell-based assay. The morphology and composition of the surface area after plasma treatment for hydrophilization purposes are stable and show constant and homogenous distribution of block nanodomains (∼22° after 4 days). These domains, which are evenly distributed on the nanoscale, therefore account for the uniform and convenient surface of a “microfluidic scale device”. To our knowledge, this is the first thermoplastic elastomer material that can be used for fast and reliable fabrication and assembly of microdevices while maintaining a high and stable hydrophilicity.
Julie Lachaux,a Clara Alcaine,b Blanca Gómez-Escoda,c Cécile M. Perrault,de David Olea Duplan,f Pei-Yun Jenny Wu,c Iñaki Ochoa,b Luis Fernandez,b Olaf Mercier,g Damien Coudreusec and Emmanuel Roy*a Author affiliations * Corresponding authors a Centre Nanosciences et Nanotechnologies, CNRS UMR9001, Paris-Saclay University, 91460 Marcoussis, France E-mail: email@example.com b Aragón Institute of Engineering Research (I3A), University of Zaragoza, Biomedical Research Network Center in Bioengineering, Biomaterials and Nanomedicine (CIBER BBN), Aragon Institute of Biomedical, Spain c Institut de Génétique et Développement de Rennes, CNRS UMR 6290, Rennes, France d Department of Mechanical Engineering, University of Sheffield, Sheffield, UK e Insigneo Institute of in-silico medecine, University of Sheffield, Sheffield, UK f Alphasip Inc., 44 Carretera de Fuencarral, 28108 Alcobendas, Madrid, Spain g Research and Innovation Unit, Inserm UMR-S 999, Marie Lannelongue Hospital, Paris-Saclay University, Le Plessis Robinson, France