[PNAS] Rapid phenotypic antimicrobial susceptibility testing using nanoliter arrays
Antibiotic resistance is a major global health concern that requires action across all sectors of society. In particular, to allow conservative and effective use of antibiotics clinical settings require better diagnostic tools that provide rapid determination of antimicrobial susceptibility. We present a method for rapid and scalable antimicrobial susceptibility testing using stationary nanoliter droplet arrays that is capable of delivering results in approximately half the time of conventional methods, allowing its results to be used the same working day. In addition, we present an algorithm for automated data analysis and a multiplexing system promoting practicality and translatability for clinical settings. We test the efficacy of our approach on numerous clinical isolates and demonstrate a 2-d reduction in diagnostic time when testing bacteria isolated directly from urine samples.
Jonathan Avesara, Dekel Rosenfelda, Marianna Truman-Rosentsvitb, Tom Ben-Aryea,c, Yuval Geffend, Moran Bercovicib, and Shulamit Levenberga,c,1 Author Affiliations
aFaculty of Biomedical Engineering, Technion – Israel Institute of Technology, Haifa 3200003, Israel; bFaculty of Mechanical Engineering, Technion – Israel Institute of Technology, Haifa 3200003, Israel; cRussell Berrie Nanotechnology Institute, Technion – Israel Institute of Technology, Haifa 3200003, Israel; dMicrobiology Laboratory, Rambam Health Care Campus, Haifa 3109601, Israel Edited by Robert Langer, Massachusetts Institute of Technology, Cambridge, MA, and approved June 1, 2017 (received for review March 6, 2017)