[Colloids and Surfaces B: Biointerfaces] Magnetic particles for in vitro molecular diagnosis: From s
Colloidal magnetic particles (MPs) have been developed in association with molecular diagnosis for several decades. MPs have the great advantage of easy manipulation using a magnet. In nucleic acid detection, these particles can act as a capture support for rapid and simple biomolecule separation. The surfaces of MPs can be modified by coating with various polymer materials to provide functionalization for different applications. The use of MPs enhances the sensitivity and specificity of detection due to the specific activity on the surface of the particles. Practical applications of MPs demonstrate greater efficiency than conventional methods. Beyond traditional detection, MPs have been successfully adopted as a smart carrier in microfluidic and lab-on-a-chip biosensors. The versatility of MPs has enabled their integration into small single detection units. MPs-based biosensors can facilitate rapid and highly sensitive detection of very small amounts of a sample. In this review, the application of MPs to the detection of nucleic acids, from sample preparation to analytical readout systems, is described. State-of-the-art integrated microsystems containing microfluidic and lab-on-a-chip biosensors for the nucleic acid detection are also addressed.
Tienrat Tangchaikeereea, b, Duangporn Polpanichc, Abdelhamid Elaissaria, Kulachart Jangpatarapongsab, , a University Lyon-1, CNRS, LAGEP UMR 5007,43 Boulevard du 11 Novembre 1918, 69100, Villeurbanne, France b Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand c National Nanotechnology Center, National Science and Technology Development Agency (NSTDA),130 Thailand Science Park, Phahonyothin Road, Khlong Luang, Pathum Thani 12120, Thailand Received 21 March 2017, Revised 5 June 2017, Accepted 19 June 2017, Available online 22 June 2017