[Analyst] A high throughput microfluidic platform for size-selective enrichment of cell populations
Numerous applications in biology and medicine require the efficient and reliable separation of cells for disease diagnosis, genetic analysis, drug screening, and therapeutics. In this work, we demonstrate a novel technology that integrates a passive and an active device to separate, enrich and release cells on-demand from a complex blood sample, or cancer cells derived from a tissue biopsy. We exploit the high throughput (>1 mL min−1), size-based sorting capability of the passive spiral inertial microfluidic (iMF) device to focus particles/cells towards an active lateral cavity acoustic transducer (LCAT) device for size-selective enrichment. We demonstrate that this platform is capable of efficiently (>90%) removing smaller cells, such as RBCs in a blood sample or smaller cancer cells in a heterogeneous cell line, and providing 44000× enrichment from the remaining sample within 5 min of device operation. Finally, we use this platform for two applications: selective enrichment of the side-population of DU-145 cells from tissue biopsy and isolation of larger monocytes from blood. Our platform integrates the high throughput (processing rate) capacity of spiral iMF with the high selectivity of LCAT, thereby offering a unique route for highly-selective, label-free particle/cell sorting, with potential application in lab-on-chip platforms for liquid biopsy and diagnostics applications.
Nivedita Nivedita,a Neha Garg,bc Abraham P. Leebcd and Ian Papautsky*ce Author affiliations * Corresponding authors a Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, USA b BioMiNT Lab, Biomedical Engineering Department, University of California, Irvine, USA c NSF Center for Advanced Design and Manufacturing of Integrated Microfluidics (CADMIM) E-mail: email@example.com d Mechanical & Aerospace Engineering Department, University of California, Irvine, USA e Department of Bioengineering, University of Illinois at Chicago, Chicago, USA