[Biomicrofluidics] Efficient extraction of oil from droplet microfluidic emulsions
Droplet microfluidic techniques can perform large numbers of single molecule and cell reactions but often require controlled, periodic flow to merge, split, and sort droplets. Here, we describe a simple method to convert aperiodic flows into periodic ones. Using an oil extraction module, we efficiently remove oil from emulsions to readjust the droplet volume fraction, velocity, and packing, producing periodic flows. The extractor acts as a universal adaptor to connect microfluidic modules that do not operate under identical flow conditions, such as droplet generators, incubators, and merger devices.
J. R. Haliburton1,2,a), S. C. Kim1,a), I. C. Clark1, R. A. Sperling3,b), D. A. Weitz3, and A. R. Abate1,2,c)lessHide Affiliations 1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, California 94158, USA 2Integrative Program in Quantitative Biology (iPQB) Biophysics Graduate Program, University of California, San Francisco, California 94158, USA 3Department of Physics and School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA a)J. R. Haliburton and S. C. Kim contributed equally to this work.
b)Present address: Fraunhofer ICT-IMM, Carl-Zeiss-Str. 18-20, 55129 Mainz, Germany.
c)Author to whom correspondence should be addressed: firstname.lastname@example.org.
Biomicrofluidics 11, 034111 (2017); doi: http://dx.doi.org/10.1063/1.4984035