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[Biomicrofluidics] Acetylated bovine serum albumin differentially inhibits polymerase chain reaction


Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA. This result is noticed for the first time, and the differential inhibition generally goes un-noticed, as compared to complete PCR inhibition.

Naveen Ramalingam1,a),b), Majid Ebrahimi Warkiani2, and Thomas Hai-Qing Gong1,a)Hide Affiliations 1School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, Singapore 639798 2School of Mechanical and Manufacturing Engineering, Australian Center for NanoMedicine, University of New South Wales, 2052 Sydney, Australia a)Authors to whom correspondence should be addressed: naveensma@gmail.com and mhqgong@ntu.edu.sg.

b)Present address: Fluidigm Corporation, 7000 Shoreline Court, 94080 South San Francisco, USA.

Biomicrofluidics 11, 034110 (2017); doi: http://dx.doi.org/10.1063/1.4983692

Link: http://aip.scitation.org/doi/abs/10.1063/1.4983692

#06192017 #labonachip #PCR #microdevice

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