[Lab on a chip] Droplet-based light-sheet fluorescence microscopy for high-throughput sample prepara
We report a novel fusion of droplet microfluidics and light-sheet microscopy, to achieve high-throughput sample compartmentalization, manipulation and three-dimensional imaging on a chip. This optofluidic device characterized by orthogonal plane illumination and rapid liquid handling is compact and cost-effective, and capable of preparing sample droplets with tunable size, frequency and ingredient. Each droplet flowing through the device's imaging region is self-scanned by a laser-sheet, three-dimensionally reconstructed and quantitatively analysed. This simple-and-robust platform combines fast 3-D imaging with efficient sample preparation and eliminates the need of a complicated mechanical scan at the same time. Achieving 500 measurements per second and screening over 30 samples per minute, it shows great potential for various lab-on-a-chip biological studies, such as embryo sorting and cell growth assays.
Hao Jiang,c Tingting Zhu,a Hao Zhang,a Jun Nie,a Zeyi Guan,b Chi-Ming Ho,b Sheng Liu*cd and Peng Fei*ab Author affiliations * Corresponding authors a School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, China E-mail: email@example.com b Mechanical and Aerospace Engineering, University of California, Los Angeles, USA c School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan, China d School of Power and Mechanical Engineering, Wuhan University, Wuhan, China