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[Sensors] Rapid Waterborne Pathogen Detection with Mobile Electronics


Abstract:

Pathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal–oxide–semiconductor (CMOS) imagers from mobile electronics, a low-cost and one-step reaction detection protocol is developed to enable field detection for waterborne pathogens. 10 mL of pathogen-containing water samples was processed using the developed protocol including filtration enrichment, immune-reaction detection and imaging processing. The limit of detection of 10 E. coli O157:H7 cells/10 mL has been demonstrated within 10 min of turnaround time. The protocol can readily be integrated into a mobile electronics such as smartphones for rapid and reproducible field detection of waterborne pathogens. View Full-Text

Tsung-Feng Wu 1,* , Yu-Chen Chen 1, Wei-Chung Wang 1, Ashwini S. Kucknoor 2, Che-Jen Lin 2OrcID, Yu-Hwa Lo 3, Chun-Wei Yao 2 and Ian Lian 2,* 1 VOR, Inc., San Diego 92122, CA, USA 2 Center for Advances in Water & Air Quality, Lamar University, Beaumont, TX 77710, USA 3 Department of Electrical and Computer Engineering, University of California at San Diego, San Diego, CA 92093, USA * Authors to whom correspondence should be addressed. Academic Editor: Jean-Louis Marty Received: 16 April 2017 / Revised: 28 May 2017 / Accepted: 7 June 2017 / Published: 9 June 2017

Link: http://www.mdpi.com/1424-8220/17/6/1348

#06142017 #pathogen #detection #MEMS #sensor #biosensor

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