[Lab on a chip] Organs-on-chips with integrated electrodes for trans-epithelial electrical resistanc
Trans-epithelial electrical resistance (TEER) is broadly used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured under static conditions in vitro, however, there is no standard methodology for its application to microfluidic organ-on-a-chip (organ chip) cultures. Here, we describe a new microfluidic organ chip design that contains embedded electrodes, and we demonstrate its utility for assessing formation and disruption of barrier function both within a human lung airway chip lined by a fully differentiated mucociliary human airway epithelium and in a human gut chip lined by intestinal epithelial cells. These chips with integrated electrodes enable real-time, non-invasive monitoring of TEER and can be applied to measure barrier function in virtually any type of cultured cell.
Olivier Y. F. Henry,ab Remi Villenave,a Michael J. Cronce,a William D. Leineweber,a Maximilian A. Benza and Donald E. Ingber*abc Author affiliations * Corresponding authors a Wyss Institute for Biologically Inspired Engineering, Harvard University, CLSB5, 3 Blackfan Circle, Boston, USA E-mail: email@example.com Web: www.wyss.harvard.edu Fax: +617 432 7828 Tel: +617 432 7044 b Harvard John A. Paulson School of Engineering and Applied Sciences, Cambridge, USA c Vascular Biology Program and Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, USA