[Lab on a chip] An automated and portable microfluidic chemiluminescence immunoassay for quantitativ
Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.
Binfeng Hu,ab Juanjuan Li,a Lei Mou,ab Yong Liu,ab Jinqi Deng,ab Wei Qian,ab Jiashu Sun,*ab Ruitao Cha*a and Xingyu Jiang*ab Author affiliations * Corresponding authors a CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China E-mail: email@example.com, firstname.lastname@example.org, email@example.com Fax: +86 10 82545631 Tel: +86 10 82545558 b University of Chinese Academy of Sciences, Beijing 100049, P. R. China