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[Biomedical Microdevices] Nano-Calorimetry based point of care biosensor for metabolic disease manag


Abstract:

Point of care (POC) diagnostics represents one of the fastest growing health care technology segments. Developments in microfabrication have led to the development of highly-sensitive nanocalorimeters ideal for directly measuring heat generated in POC biosensors. Here we present a novel nano-calorimeter-based biosensor design with differential sensing to eliminate common mode noise and capillary microfluidic channels for sample delivery to the thermoelectric sensor. The calorimeter has a resolution of 1.4 ± 0.2 nJ/(Hz)1/2 utilizing a 27 junction bismuth/titanium thermopile, with a total Seebeck coefficient of 2160 μV/K. Sample is wicked to the calorimeter through a capillary channel making it suitable for monitoring blood obtained through a finger prick (<1 μL sample required). We demonstrate device performance in a model assay using catalase, achieving a threshold for hydrogen peroxide quantification of 50 μM. The potential for our device as a POC blood test for metabolic diseases is shown through the quantification of phenylalanine (Phe) in serum, an unmet necessary service in the management of Phenylketonuria (PKU). Pegylated phenylalanine ammonia-lyase (PEG-PAL) was utilized to react with Phe, but reliable detection was limited to <5 mM due to low enzymatic activity. The POC biosensor concept can be multiplexed and adapted to a large number of metabolic diseases utilizing different immobilized enzymes.

Evan KazuraBrad R. LubbersElliott DawsonJohn A. PhillipsIIIFranz BaudenbacherEmail author First Online: 31 May 2017 DOI: 10.1007/s10544-017-0181-4 Cite this article as: Kazura, E., Lubbers, B.R., Dawson, E. et al. Biomed Microdevices (2017) 19: 50. doi:10.1007/s10544-017-0181-4

Link: https://link.springer.com/article/10.1007%2Fs10544-017-0181-4

#06032017 #biosensor #pointofcare #microchannels

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