[Colloids and Surfaces B: Biointerfaces] High-throughput continuous production of liposomes using hy
The microfluidic hydrodynamic flow-focusing is a simple technique for nanoscale liposome formation that provides several advantages compared to the traditional manufacturing techniques. This work aimed to perform a systematic study of the liposome formation using planar microfluidic devices with different channel aspect-ratios, as an alternative to enhance the throughput of liposome synthesis. In general, liposomes with a low polydispersity and a precise control of the size were successfully produced from alteration of the flow rate ratio and channel aspect-ratio. The higher aspect-ratio enabled the most rapid generation of liposomes with similar diameter and significant lower polydispersity index than the obtained by other batch technique. Besides, β-carotene was successfully incorporated into liposomes with efficiency of approximately 60% and the incorporation ability was not specific to a choice of microfluidic device aspect-ratio. The results suggest that the use of microfluidic devices could be employed for liposome production with a possible advantage to minimize the degree of parallelization of processes. These results demonstrate the potential technical feasibility of microfluidic processes for future industrial applications.
Mariano Michelon1, , Davi Rocha Bernardes Oliveira1, , Guilherme de Figueiredo Furtado1, , Lucimara Gaziola de la Torre2, , Rosiane Lopes Cunha1, , 1 Department of Food Engineering, School of Food Engineering, University of Campinas, Campinas 13083862, SP, Brazil 2 Department of Materials and Bioprocess Engineering, School of Chemical Engineering, University of Campinas, Campinas 13083852, SP, Brazil Received 14 February 2017, Accepted 10 May 2017, Available online 11 May 2017
Show less https://doi.org/10.1016/j.colsurfb.2017.05.033