[ACS Nano] Platelet-Microcapsule Hybrids Leverage Contractile Force for Targeted Delivery of Hemosta
We report a cell-mediated, targeted drug delivery system utilizing polyelectrolyte multilayer capsules that hybridize with the patient’s own platelets upon intravenous administration. The hybridized platelets function as the sensor and actuator for targeted drug delivery and controlled release in our system. These capsules are biochemically and mechanically tuned to enable platelet adhesion and capsule rupture upon platelet activation and contraction, enabling the targeted and controlled “burst” release of an encapsulated biotherapeutic. As platelets are the “first responders” in the blood clot formation process, this platelet-hybridized system is ideal for the targeted delivery of clot augmenting biotherapeutics wherein immediate therapeutic efficacy is required. As proof-of-concept, we tailored this system to deliver the pro-clotting biotherapeutic, factor VIII for hemophilia A patients that have developed inhibitory anti-factor VIII antibodies. The polyelectrolyte multilayer capsules physically shield the encapsulated factor VIII from the patient’s inhibitors during circulation, preserving its bioactivity until it is delivered at the target site via platelet contractile force. Using an in vitro microfluidic vascular injury model with factor VIII-inhibited blood, we demonstrate a 3.8x increase in induced fibrin formation using capsules loaded with factor VIII at a concentration an order of magnitude lower than that used in systemic delivery. We further demonstrate that clot formation occurs 18 minutes faster when factor VIII loaded capsules are used compared to systemic delivery at the same concentration. Because platelets are integral in the pathophysiology of thrombotic disorders, cancer, and innate immunity, this paradigm-shifting smart drug delivery system can be similarly applied to these diseases.
Caroline E. Hansen, David R. Myers, Wallace Hunter Baldwin, Yumiko Sakurai, Shannon L. Meeks, L. Andrew Lyon, and Wilbur A. Lam
ACS Nano, Just Accepted Manuscript
Publication Date (Web): May 25, 2017
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