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[Biotechnology Letters] Three-dimensional co-culture microfluidic model and its application for rese



To build a three-dimensional co-culture model in a microfluidic device for cancer research and evaluate its feasibility by investigating cancer stem-like cells (SCs) induced migration of human umbilical vein endothelial cells (ECs).


The microfluidic device provided two-dimensional and three-dimensional (2D/3D) culture and co-culture environments without affecting cell viability. The device also provided an effective concentration for the chemiotaxis of cells, and to support real-time monitoring of cell behavior. In this model, SCs significantly increased the migration area of ECs with a hepatocarcinoma cell line (MHCC97H; MCs). The presence of ECs also induced both MCs and SCs invasion into Matrigel. The migration area of MCs and SCs significantly increased when co-cultured with ECs.


This 3D co-culture microfluidic model is a suitable model in cancer research. Compared with MCs, SCs had greater potential in inducing EC migration and interacting with ECs.

Yi Zhao, Xiaoqing Yan, Bo Li, Ming Ke, Sijia Chen, Zhiling Xu, Shaoxi Cai First Online: 23 May 2017 DOI: 10.1007/s10529-017-2363-9 Cite this article as: Zhao, Y., Yan, X., Li, B. et al. Biotechnol Lett (2017). doi:10.1007/s10529-017-2363-9

Link: https://link.springer.com/article/10.1007%2Fs10529-017-2363-9

#05272017 #cell #Biologicalapplication #labonachip

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