[Colloids and Surfaces B: Biointerfaces] Controlled release of moxifloxacin from intraocular lenses
Intraocular lenses (IOLs) present an alternative for extended, local drug delivery in the prevention of post-operative acute endophthalmitis. In the present work, we modified the surface of a hydrophilic acrylic material, used for manufacturing of IOLs, through plasma-assisted grafting copolymerization of 2-acrylamido-2-methylpropane sulfonic acid (AMPS) or [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (SBMA), with the aim of achieving a controlled and effective drug release. The material was loaded with moxifloxacin (MFX), a commonly used antibiotic for endophthalmitis prevention. The characterization of the modified material showed that relevant properties, like swelling capacity, wettability, refractive index and transmittance, were not affected by the surface modification. Concerning the drug release profiles, the most promising result was obtained when AMPS grafting was done in the presence of MFX. This modification led to a higher amount of drug being released for a longer period of time, which is a requirement for the prevention of endophthalmitis. The material was found to be non-cytotoxic for rabbit corneal endothelial cells. In a second step, prototype IOLs were modified with AMPS and loaded with MFX as previously and, after sterilization and storage (30 days), they were tested under dynamic conditions, in a microfluidic cell with volume and renovation rate similar to the eye aqueous humour. MFX solutions collected in this assay were tested against Staphylococcus aureus and Staphylococcus epidermidis and the released antibiotic proved to be effective against both bacteria until the 12th day of release.
A.F.R. Pimentaa, b, A.P. Vieirac, R. Colaçob, B. Saramagoa, M.H. Gilc, P. Coimbrac, P. Alvesc, D. Bozukovad, T.R. Correiae, I.J. Correiae, A.J. Guiomarc, f, A.P. Serroa, g, , a CQE, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal b IDMEC, Departamento de Engenharia Mecânica, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal c CIEPQPF, Departamento de Engenharia Química, Universidade de Coimbra, Coimbra, Portugal d CICS-UBI, Liège, Belgium e CICS, Departamento de Ciências Médicas, Universidade da Beira Interior, Covilhã, Portugal f Departamento de Ciências da Vida, Universidade de Coimbra, Coimbra, Portugal g CIIEM, Instituto Superior de Ciências da Saúde Egas Moniz, Caparica, Portugal Received 23 January 2017, Revised 3 April 2017, Accepted 29 April 2017, Available online 1 May 2017