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[Analytical Chemistry] Preparation of Linear Cryogel Arrays as a Microfluidic Platform for Immunochr


Abstract:

We describe a new microfluidic platform to perform immunochromatographic assays. The platform consists of a linear assembly of small, porous cryogel monoliths functionalized with different biomolecules and anchored in an optically transparent capillary, which serves as the microfluidic carrier. This assembly enables fluid flow by capillary action and simple optical detection. Using an in situ preparation method, individual compartments are generated from small plugs of polymer solutions that are subsequently transformed into individually functionalized cryogel monoliths through a photoinduced cross-linking reaction. In the same reaction step, the monoliths are firmly anchored to the surface of the capillary. A proof-of-concept, prototype platform is successfully used for the detection of the inflammatory marker interleukin 6 via a sandwich immunoassay. We observe excellent assay performance metrics that include high sensitivity, good linearity, and low variation. We also demonstrate fluid transport solely by passive means, which is a critical attribute for point-of-care diagnostics.

Marc Zinggeler , Patrick L. Fosso, Yan Hao, Thomas Brandstetter, and Jürgen Rühe* Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany Anal. Chem., Article ASAP DOI: 10.1021/acs.analchem.7b01182 Publication Date (Web): May 22, 2017 Copyright © 2017 American Chemical Society *E-mail: ruehe@imtek.de.

Link: http://pubs.acs.org/doi/abs/10.1021/acs.analchem.7b01182

#05242017 #chemistry #hydrogel #immunoassay

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