[Biosensors and Bioelectronics] A Point-of-Need infrared mediated PCR platform with compatible later
With the increasing need of monitoring the epidemiology of serious infectious diseases, food hygiene, food additives and pesticide residues, it is urgent to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. Herein, we demonstrate a prototype of IR mediated Conducting Oil and CarbOn Nanotube circUlaTing PCR (IR-COCONUT PCR) platform for nucleic acid amplification. The presented platform offers a new solution for miniaturized PCR instruments with non-contact heaters by using conducting oil and carbon nanotube as a medium in IR mediated PCR. This novel platform offers accurate and flexible control of temperature through the integration of PID (proportional–integral–derivative) algorithms to manipulate the duty cycle of the voltage signals of IR LED and a peristaltic pump. The ramping rate of the introduced platform in current study is 1.5 °C/s for heating speed and −2.0 °C/s for cooling speed. This platform fulfills 30 thermal cycles within 50 min which is a match to the conventional bench-top PCR thermo cyclers. For demonstration purpose, human papillomavirus (HPV) patient cervical swab specimens were examined. Downstream lateral flow strip (LFS) was also developed to quantity the PCR products from the IR-COCONUT PCR device within 25 min. This PCR platform together with the compatible LFS shows great potential for in-field and Point-of-Need (PoN) testing of genetic or contagious diseases.
Wenjia Liua, Mingfang Zhangb, Xiaoyan Liua, Alok Sharmaa, Xianting Dinga, , a State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China b Gene Company Limited, Jingzhuang business center, No.772 Yingtian Street, Jianye District, Nanjing 210000, China Received 25 January 2017, Revised 26 April 2017, Accepted 27 April 2017, Available online 29 April 2017