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[Langmuir] Strategically designing a pumpless microfluidic device on an “inert” polypropylene substr


This study is an attempt to make a step forward to implement the very immature concept of pumpless transportation of liquid into a real miniaturized device or lab-on-chip (LOC) on a plastic substrate. “Inert” plastic materials such as polypropylene (PP) are used in a variety of biomedical applications but their surface engineering is very challenging. Here, it was demonstrated that with a facile innovative wettability patterning route using fluorosilanized UV-independent TiO2 nanoparticle coating it is possible to create wedge-shape open microfluidic tracks on inert solid surfaces for low-cost biomedical devices (Lab-on-plastic). For the future miniaturization and integration of the tracks into a device, a variety of characterization techniques were used to not only systematically study the surface patterning chemistry and topography but also to have a clear knowledge of its biological interactions and performance. The effect of such surface architecture on the biological performance was studied in terms of static/dynamic protein (bovine serum albumin) adsorption, bacterial (staphylococcus aureus and staphylococcus epidermidis) adhesion, cell viability (using HeLa and MCF-7 cancer cell lines as well as non-cancerous human fibroblast cells), and cell patterning (Murine embryonic fibroblasts). Strategies for incorporating such a confined track into a diagnostic device which its sensing portion is based on protein, microorganism, or cells are discussed. Finally, for the proof-of-principle of biosensing application, the well-known high-affinity molecular couple of BSA-antiBSA as a biological model was employed.

Elham Shirani, Amir Razmjou, Hossein Tavassoli, Amir Landarani-Isfahani, Saghar Rezaei, Abolghasem Abbasi Kajani, Mohsen Asadnia, Jingwei Hou, and Majid Ebrahimi Warkiani

Langmuir, Just Accepted Manuscript

DOI: 10.1021/acs.langmuir.7b00537

Publication Date (Web): May 10, 2017

Copyright © 2017 American Chemical Society

Link: http://pubs.acs.org/doi/abs/10.1021/acs.langmuir.7b00537

#05132017 #biosensing #diagnostics #labonachip

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