Abstract:

We propose a strategy for real time multicolour detection of light emitting particles under flow conditions, using the diffracted orders of a grating. The method relies on the fact that the spatial (angular/linear) separation between the orders of a diffraction grating is a wavelength dependent quantity. Thus, the difference in arrival times at a fixed detector for neighbouring orders produced by a moving, light-emitting particle can be used as a wavelength sensing mechanism. We demonstrate this functionality using a prototype PDMS microfluidic device that incorporates a focusing transmission grating. A key attribute of this approach is that it requires only a single sensitive detector, such as a photomultiplier tube, in contrast to many conventional approaches which require multiple sensitive detectors or a detector array.

Seyed M. Azmayesh-Fard,a Aaron Melnyk,a Lawrence Lam,b Trevor Allen,a Robert Fedosejevsa and Ray G. DeCorby*a

Author affiliations

* Corresponding authors

a Department of Electrical and Computer Engineering, University of Alberta, ECERF Building, 9107 - 116 Street, N.W., Edmonton, Canada E-mail: rdecorby@ualberta.ca Tel: +1 780 492 5585

b Innovation Center, National Institute for Nanotechnology (NINT), 11421 Saskatchewan Drive, Edmonton, Canada

Link: http://pubs.rsc.org/en/Content/ArticleLanding/2017/LC/C7LC00259A#!divAbstract

#05132017 #sensing #Biologicalapplication #grating #detector #labonachip