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[Biofabrication] Leaf-inspired microcontact printing vascular patterns


The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature. By coating the reverse mold with 50µg/ml of fibronectin and stamping enabled selective adhesion of the human umbilical vein endothelial cells (HUVECS) to the patterned adhesive matrix, we show that a vascular-branching pattern can be transferred by microcontact printing. Moreover, this pattern can be maintained transferred to a 3D hydrogel matrix and remains stable for up to 4 days. After 4 days, HUVECs can be observed migrating and sprouting into Matrigel. These printed vascular branching patterns, especially after transfer to 3D hydrogels, provide a viable alternative strategy to the prevascularization of complex tissues.

Lian Wong1, John Pegan2, Basia Gabela-Zuniga3, Michelle Khine4 and Kara E McCloskey5 Accepted Manuscript online 10 May 2017 • © 2017 IOP Publishing Ltd

Link: http://iopscience.iop.org/article/10.1088/1758-5090/aa721d

#05132017 #microfabrication #tissueengineering #cell #Biologicalapplication #hydrogel

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