[Experimental Cell Research] The effects of activin A on the migration of human breast cancer cells
Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the effect of activin A on the migration of human breast cancer cell line MDA-MB-231 cells and human blood neutrophils as well as their migratory interaction. We found that activin A promoted the basal migration but impaired epidermal growth factor (EGF)-induced migration of breast cancer cells. By contrast, activin A reduced neutrophil chemotaxis and transendothelial migration to N-Formyl-Met-Leu-Phe (fMLP). Finally, activin A promoted neutrophil chemotaxis to the supernatant from breast cancer cell culture. Collectively, our study revealed the different roles of activin A in regulating the migration of breast cancer cells and neutrophils and their migratory interaction. These findings suggested the potential of activin A as a therapeutic target for inflammation and breast cancers.
Dongxue Xiea, b, 1, Zhonghui Liuc, 1, Jiandong Wub, Wenfang Fenga, Ke Yangb, d, Jixian Denge, Ganghong Tiane, Susy Santosf, Xueling Cuia, b, , , Francis Linb, , a Department of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, 130021, P.R. China b Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada c Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, P.R. China d Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, P.R. China e National Research Council, Winnipeg, MB, R3B 1Y6, Canada f Victoria General Hospital and River Heights/Fort Garry Community areas, Winnipeg, MB, Canada Received 5 March 2017, Revised 29 April 2017, Accepted 3 May 2017, Available online 4 May 2017