[Biofabrication] Synthesis of cell composite alginate microfibers by microfluidics with the applicat
Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application remains is still challenging. In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the methods of microfluidics were considered original. A simple glass microfluidic device with a "L-shape" cylindrical-flow channel in the microfluidic platform was developed. The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow respectively. The diameters of the microfibers could be controlled by varying flow rates and the glass capillary tubes diameters at their tips. The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts. The encapsulated mesenchymal stem cells (MSCs) proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor (VEGF) and fibroblastic growth factor (FGF). The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction. These encouraging results suggest great promise of the application of microfluidics as future alternative in SDVGs engineering.
Mingying Liu1, Zhizhi Zhou2, Yongchao Chai3, Shuai Zhang4, Xiaomeng Wu5, Shaopeng Huang6, Jiaqiang Su7 and Jiahuan Jiang8 Accepted Manuscript online 9 May 2017 • © 2017 IOP Publishing Ltd