[ IEEE Transactions on Components, Packaging and Manufacturing Technology] A Digital Signal Processi
We report the use of digital signal processing (DSP) strategies to realize high-sensitivity on-chip fluorescence detection in microfluidic-opto-electronic printed circuit board (PCB) devices without requiring bulky high-quality optical components and filters. An isotachophoresis (ITP) assay was studied in a microfluidic PCB with an embedded standard complementary metal oxide semiconductor sensor array demonstrating a detection sensitivity of 10-nm initial concentration for fluorescein. The amplified ITP signal is processed with the DSP algorithms allowing the fluorescence signal extracted from noisy sensor output to achieve high signal-to-noise ratio. The DSP-assisted fluorescence detection system utilizes off-the-shelf, consumer-grade electronics and lensless imaging to achieve high-level but also scalable integration on microfluidic PCBs.
Sarkis Babikian University of California Irvine, Irvine CA 92697 USA. G. P. Li University of California Irvine, Irvine CA 92697 USA. Mark Bachman University of California Irvine, Irvine CA 92697 USA. He is now with Integra Devices, Irvine CA 92697 USA.
Published in: IEEE Transactions on Components, Packaging and Manufacturing Technology ( Volume: PP, Issue: 99 ) Page(s): 1 - 9 Date of Publication: 02 May 2017 ISSN Information: Print ISSN: 2156-3950 Online ISSN: 2156-3985 DOI: 10.1109/TCPMT.2017.2691673 Publisher: IEEE