[Biosensors and Bioelectronics] A bead-based immunofluorescence-assay on a microfluidic dielectropho
The proof of concept of utilizing a microfluidic dielectrophoresis (DEP) chip was conducted to rapidly detect a dengue virus (DENV) in vitro based on the fluorescence immunosensing. The mechanism of detection was that the DEP force was employed to capture the modified beads (mouse anti-flavivirus monoclonal antibody-coated beads) in the microfluidic chip and the DENV modified with fluorescence label, as the detection target, can be then captured on the modified beads by immunoreaction. The fluorescent signal was then obtained through fluorescence microscopy, and then quantified by ImageJ freeware. The platform can accelerate an immuno-reaction time, in which the on-chip detection time was 5 min, and demonstrating an ability for DENV detection as low as 104 PFU/mL. Furthermore, the required volume of DENV samples dramatically reduced, from the commonly used ~50 µL to ~15 µL, and the chip was reusable (>50x). Overall, this platform provides a rapid detection (5 min) of the DENV with a low sample volume, compared to conventional methods. This proof of concept with regard to a microfluidic dielectrophoresis chip thus shows the potential of immunofluorescence based-assay applications to meet diagnostic needs.
Edwar Iswardya, b, Tien-Chun Tsaia, I-Fang Chengc, Tzu-Chuan Hod, e, Guey Chuen Perngd, e, f, , 1, , Hsien-Chang Changa, g, , 1, a Institute of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan b Department of Physics, Syiah Kuala University, Banda Aceh 23111, Indonesia c National Nano Device Laboratories, National Applied Research Laboratories, Tainan 70101, Taiwan d Department of Microbiology and Immunology, National Cheng Kung University, Tainan 70101, Taiwan e Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan f Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70101, Taiwan g Medical Device Innovation Center, National Cheng Kung University, Tainan 70101, Taiwan Received 24 February 2017, Revised 7 April 2017, Accepted 11 April 2017, Available online 12 April 2017