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[SPIE] Quantitative phase microscopy of cells in flow using flipping interferometry


Measurements of biological cells during flow are highly important for medical diagnosis based on cell sorting. In the case of cell imaging during flow, very rapid image acquisition capabilities are required to enable fast cell flow for analyzing a sufficient number of cells. We present a new flipping interferometry (FI) module for simplified off-axis close-to-common-path interferometric phase microscopy. This wide-field off-axis interferometric module provides rapid quantitative phase microscopy of biological cells during flow in a microfluidic channel, with potential of integration into cell sorting devices. Various experimental demonstrations are presented. © (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Natan T. Shaked ; Bahram Javidi ; Nir A. Turko ; Darina Roitshtain [-] Author Affiliations Natan T. Shaked, Nir A. Turko, Darina Roitshtain Tel Aviv Univ. (Israel)

Bahram Javidi Univ. of Connecticut (United States)

Proc. SPIE 10074, Quantitative Phase Imaging III, 1007411 (April 24, 2017); doi:10.1117/12.2252341

Link: http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2624131

#05012017 #interferometry #Biologicalapplication #cell #microchannels

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