[Frontiers in Bioengineering and Biotechnology] Microfluidic Concentric Gradient Generator Design fo
Gradients of diffusible signaling molecules play important role in various processes, ranging from cell differentiation to toxicological evaluation. Microfluidic technology provides an accurate control of tempospatial conditions. However, current microfluidic platforms are not designed to handle multiple gradients and cell populations simultaneously. Here, we demonstrate a rapidly adaptable microfluidic design able to expose multiple cell populations to an array of chemical gradients. Our design is based on pressure-equilibrated concentric channels and a pressure-dissipating control layer, facilitating the seeding of multiple cell populations in a single device. The design was numerically evaluated and experimentally validated. The device consists of 8 radiating stimuli channels and 12 circular cell culture channels, creating an array of 96 different continuous gradients that can be simultaneously monitored over time.
Elishai Ezra Tsur1,2, Michal Zimerman1, Idan Maor1, Avner Elrich1 and Yaakov Nahmias1,3* 1Grass Center for Bioengineering, The Hebrew University of Jerusalem, Jerusalem, Israel 2Neuro-Biomorphic Engineering Lab, Faculty of Engineering, Jerusalem College of Technology, Jerusalem, Israel 3Department of Cell and Developmental Biology, The Hebrew University of Jerusalem, Jerusalem, Israel