[Lab on a chip] Fabrication of large-area polymer microfilter membranes and their application for pa
A vacuum assisted UV micro-molding (VAUM) process is proposed for the fabrication of freestanding and defect-free polymer membranes based on a UV-curable methacrylate polymer (MD 700). VAUM is a highly flexible and powerful method for fabricating low cost, robust, large-area membranes over 9 × 9 cm2 with pore sizes from 8 to 20 μm in diameter, 20 to 100 μm in thickness, high aspect ratio (the thickness of the polymer over the diameter of the hole is up to 15:1), high porosity, and a wide variety of geometrical characteristics. The fabricated freestanding membranes are flexible while mechanically robust enough for post manipulation and handling, which allows them to be cut and integrated as a plastic cartridge onto thermoplastic 3D microfluidic devices with single or double filtration stages. Very high particle capture efficiencies (≈98%) have been demonstrated in the microfluidic devices integrated with polymer membranes, even when the size of the beads is very close to the size of the pores of the microfilter. About 85% of the capture efficiency has been achieved in cancer cell trapping experiments, in which a breast cancer cell line (MDA-MB-231) spiked with phosphate-buffered saline buffer when the pore size of the filter is 8 μm and the device is operated at a flow rate of 0.1 mL min−1.
Javier Alejandro Hernández-Castro, abc Kebin Li, a Anne Meunier, bc David Juncker bcd and Teodor Veres *ab Author affiliations * Corresponding authors a National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, Québec, J4B 6Y4 Canada E-mail: email@example.com Fax: +1 450 641 5105 Tel: +1 450 641 5232 b Biomedical Engineering Department, McGill University, 3775 University Street, Montréal, Québec, H3A 2B4 Canada c McGill University and Génome Québec Innovation Centre, McGill University, 740 Dr. Penfield Avenue, Montréal, Québec, H3A 0G1 Canada d Neurology and Neurosurgery Department, McGill University, 3801 University Street, Montréal, Québec, H3A 2B4 Canada