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[ACS Appl. Mater. Interfaces] β-CD-functionalized microdevice for rapid capture and release of bacte


Abstract:

Most procedures for detecting pathogens in liquid media require an initial concentration step. In this regard, carbohydrates have proven to be attractive affinity ligands for the solid-phase capture of bacteria that use lectins for adhesion to host cell membranes. However, the use of cyclodextrin-immobilized substrates to selectively trap bacteria has not been explored before. Here, using quartz-crystal microbalance with dissipation monitoring experiments, we demonstrate that functionalization of surfaces by β-cyclodextrin (β-CD) can not only allow for rapid and efficient capture of bacterial cells in liquid but also their facile elution with an aqueous solution of a selectively methylated β-CD derivative as a competitive molecule. This capture/elution strategy, which is based on host-guest interactions between membrane components of the bacterial cell and the CD cavities, is performed in physiological conditions and can be integrated in a microchip. Indeed, proof-of-concept studies showed the potential of β-CD-modified micropillar-integrated microfluidic devices for concentration of bacteria. The results obtained with E. coli suggest that this approach could be broadly applicable among Gram-negative bacteria, which share common cell membrane structures.

Alexandra Perez-Anes, Anna Szarpak-Jankowska, Dorothée Jary, and Rachel Auzely-Velty ACS Appl. Mater. Interfaces, Just Accepted Manuscript DOI: 10.1021/acsami.7b02194 Publication Date (Web): April 10, 2017 Copyright © 2017 American Chemical Society

Link: http://pubs.acs.org/doi/abs/10.1021/acsami.7b02194

#04112017 #capture #bacteria #membrane

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