[Analytical Chemistry] Bedside Immune Monitoring: An Automated Immunoassay Platform for Rapid Quanti
This article presents an automated, compact and self-contained system for a sensitive quantitative detection of blood biomarkers. A disposable microfluidic chip, prefilled with biomarker-specific reagents and magnetic beads, can be processed fully automatically by a readout-platform, enabling an immunoassay-based analysis with a processing time from sample incubation until signal analysis of 20 minutes. Novel concepts for on-chip vortexing of the magnetic beads and on-chip reagent storage and actuation were developed. A lens-free photodiode readout system represents a cost-efficient approach for detecting the chemiluminescent signal. IL-8 spiked serum samples were measured with a high reproducibility and a limit of detection of 2.05 pg·mL−1. The system was validated with undiluted serum samples collected from trauma patients at the intensive care unit. The developed platform demonstrated good correlation with the clinical reference method and the clinical trajectory course of IL 8 could be sufficiently followed. With an automated assay approach and an easily adaptable protocol, this portable platform has the potential to be utilized as a universal instrument for analyzing proteins in small sample volumes (<25 µL) in point-of-care settings.
Helene Zirath, Guntram Schnetz, Andreas Glatz, Andreas Spittler, Heinz Redl, and Johannes R. Peham Anal. Chem., Just Accepted Manuscript DOI: 10.1021/acs.analchem.6b03624 Publication Date (Web): April 6, 2017 Copyright © 2017 American Chemical Society