[European Journal of Pharmaceutics and Biopharmaceutics] DynaMiTES - a dynamic cell culture platform
In the present study, a formerly designed Dynamic Micro Tissue Engineering System (DynaMiTES) was applied with our prevalidated human hemicornea (HC) construct to obtain a test platform for improved absorption studies of the anterior eye (Ocular DynaMiTES). First, the cultivation procedure of the classic HC was slightly adapted to the novel DynaMiTES design. The obtained inverted HC was then compared to classic HC regarding cell morphology using light and scanning electron microscopy, cell viability using MTT dye reaction and epithelial barrier properties observing transepithelial electrical resistance and apparent permeation coefficient of sodium fluorescein. These tested cell criteria were similar. In addition, the effects of four different flow rates on the same cell characteristics were investigated using the DynaMiTES. Because no harmful potential of flow was found, dynamic absorption studies of sodium fluorescein with and without 0.005%, 0.01% and 0.02% benzalkonium chloride were performed compared to the common static test procedure. In this proof-of-concept study, the dynamic test conditions showed different results than the static test conditions with a better prediction of in vivo data. Thus, we propose that our DynaMiTES platform provides great opportunities for the improvement of common in vitro drug testing procedures.
Nicole Beißnera, c, 1, Kai Matternb, c, 1, Andreas Dietzelb, c, Stephan Reichla, c, , a Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Mendelssohnstraße 1, 38106 Braunschweig, Germany b Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Straße 203, 38124 Braunschweig, Germany c Center of Pharmaceutical Engineering – PVZ, Technische Universität Braunschweig, Franz–Liszt-Straße 35 A, 38106 Braunschweig Received 27 January 2017, Revised 27 March 2017, Accepted 29 March 2017, Available online 2 April 2017 http://doi.org/10.1016/j.ejpb.2017.03.021