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[Talanta] Determination of Escherichia coli in urine using a low-cost foil-based microfluidic device


Abstract:

We developed a simple low-cost cultivation-based microfluidic device from office-laminator foil and Parafilm for the determination of specific microorganisms in water samples. The main goal was to obtain a device that would be portable and cheap compared to common laboratory techniques testing microorganisms. This device needs only 10 µL of a sample and can be easily used in terrain by a non-specialist. Moreover, we dealt with some technical aspects of the device fabrication such as low-cost lamination techniques and the use of different cultivation media.

Tereza Mašková, Lenka Hárendarčíková, Jan Petr, Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic Received 30 January 2017, Revised 24 March 2017, Accepted 28 March 2017, Available online 30 March 2017

Show less http://dx.doi.org/10.1016/j.talanta.2017.03.089

Link: http://www.sciencedirect.com/science/article/pii/S003991401730396X

#04032017 #urine #parafilm #microorganisms #lamination #Ecoli #bacteria #foil #MEMS #labonachip

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