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[Microfluidics and Nanofluidics] A simple method of constructing microfluidic solid-state quantum do


Solid-state molecular beacons show great potential for label-free biomarker detection, however, it is challenging to construct robust and homogenous quantum dot molecular beacon microarrays in a microfluidic platform. Here, we report a simple and cost-effective method of constructing a mercaptopropionic acid quantum dot (MPA-QD) microarray in a microfluidic platform using a PDMS through-hole structure. This method combines soft lithography and surface functionalization to achieve uniform QD immobilization in a microfluidic network. With the simple fabricated quantum dot microarray sensor integrated in the microfluidic device, label-free biomarker detection was performed with high efficiency, specificity and sensitivity. We performed cardiovascular biomarker detection, and our microfluidic QD molecular beacon platform achieved target DNA sequence identification at a low concentration of 1 nM/L.

Hongwei Liu, Weiliang Shu, Zongbin Liu, Baoyue Zhang, Hongtao Feng, Yan Chen

First Online: 27 March 2017 DOI: 10.1007/s10404-017-1906-7 Cite this article as: Liu, H., Shu, W., Liu, Z. et al. Microfluid Nanofluid (2017) 21: 67. doi:10.1007/s10404-017-1906-7

Link: https://link.springer.com/article/10.1007/s10404-017-1906-7

#04012017 #quantumdots #labelfree #detection #biomarker #Biologicalapplication

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