Abstract:

There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid–liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.

Marta Sobrinos-Sanguino,a Silvia Zorrilla,*a Christine D. Keating,b Begoña Monterroso*a and Germán Rivas*a Author affiliations * Corresponding authors a Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain E-mail: silvia@cib.csic.es, monterroso@cib.csic.es, grivas@cib.csic.es b Department of Chemistry, Pennsylvania State University, University Park, USA

Link: http://pubs.rsc.org/en/content/articlelanding/2017/cc/c7cc01289f#!divAbstract

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