[BCSJ] Single Particle Tracking Reveals a Dynamic Role of Actin Filaments in Assisting Long-Range Ax
Here, we demonstrated that actin filaments mediate axonal transport in dorsal root ganglia (DRG) neurons using fluorescence single-particle tracking. We employed a compartmentalized microfluidic cell culturing chamber that allows depolymerization of actin filaments within an axonal segment. We observed that local depolymerization results in a two-fold increase in the average pausing duration, whereas the microtubule-dependent instantaneous transport speed is not perturbed. Collectively, our data reveal an important role of actin filaments in assisting microtubule-dependent long-range NGF axonal transport in DRG neurons.
Yasuko Osakada*1,2 and Kai Zhang3 DOI: http://dx.doi.org/10.1246/bcsj.20170090 Affiliations 1Department of Chemistry, Stanford University, Stanford, CA 94305 (USA) 2The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047 (Japan) 3Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, 314 B Roger Adams Laboratory, Urbana, Illinois, 61801 (USA)
Additional Info Received: March 11, 2017 Accepted: March 24, 2017 Web Released: March 29, 2017