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[Biomicrofluidics] Evaluation of disposable microfluidic chip design for automated and fast Immunoas


Abstract:

We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within ∼1 h. The unique design allows for real-time dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, and 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-the-shelf components with the final chemiluminescent detection carried out in a commercial plate reader. Optimization of different aspects of chip design, fabrication, and assay development is discussed in detail.

Guochun Wang1, Champak Das1, Bradley Ledden1, Qian Sun1, Chien Nguyen1, and Sai Kumar2 Biomicrofluidics 11, 014115 (2017); doi: http://dx.doi.org/10.1063/1.4977198

1SFC Fluidics, Inc., Fayetteville, Arkansas 72701, USA 2Diligent CXO, Norcross, Georgia 30071, USA

Link: http://aip.scitation.org/doi/10.1063/1.4977198

#03282017 #Biologicalapplication #immunoassay #biomarker #detection

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