[Patent] Non-invasive monitoring cancer using integrated microfluidic profiling of circulating micro
A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.
Publication number US20170065978 A1 Publication type Application Application number US 15/357,231 Publication date Mar 9, 2017 Filing date Nov 21, 2016 Priority date Mar 14, 2014 Inventors Zheng Zhao, Mei He, Yong Zeng Original Assignee University Of Kansas, Kansas State University Research Foundation