[Food Control] Auto-microfluidic thin-film chip for genetically modified maize detection
As a thin-film chip method, reverse dot blot hybridization (RDBH) has been employed to detect hazardous substances, but an automatic RDBH instrument with low workload, high accuracy and stability is still urgently needed. This paper presents our newly-developed auto-microfluidic thin-film chip (AMTC) method for multiplex screening of genetically modified (GM) maize. With specific DNA probes for genetically modified (GM) maize being immobilized on a square nylon thin-film, it was placed into a micro-reaction cell of the AMTC device. Then biotin-labeled PCR products with target DNA fragments for template amplification were added to the micro-reaction cell using a microfluidic system. When the PCR products passed the the square nylon thin-film, the target DNA fragments were captured by the complementary action of DNA, where the signal was visualized with streptavidin link-coupled alkaline phosphatase color development kit. The sensitivity of GM maize detection reached 0.1% quality percentage and its stability and consistency could satisfy the requirements for practical applications. Performance advantages of the ATMC are manifold, being embodied in aspects such as easy and straightforward operation, low costs and less workload.
Zhenyu Yunb, Liping Pengc, Xin Wud, Shengnan Huoe, Jun Zhoua, Yuanhong Denga, Wenjing Lia, Cai Qif, Guangping Huanga, , a Sichuan Hua Hansan Bio Technology Co., Ltd. #39, Fucheng West Rd., Chengdu, 610041, China b China National Institute of Standardization, #4, Zhichun Rd., Haidian District, Beijing 100088, China c National Center of Agriculture Standardization Monitoring and Researching, #1218 Chuangxin 2nd Rd., Songbei science and technology District, Harbin 150028, China d Jiangxi Institute for Food Control, #69 Beijing West Rd., Donghu District, Nanchang 330001,China e Shandong Institute for Food and Drug Control, #2749 Xinluo Rd., Gaoxin District, Jinan 250101,China f Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, #3, Gaobeidian North Rd., Beijing, 100123,China Available online 24 March 2017