[Patent] Droplet Microfluidic Device and Methods of Sensing the results of an assay therein
A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.
Document Type and Number: United States Patent Application 20170056887 Kind Code: A1
Inventors: Hadwen, Benjamin James (Oxford, GB) Jacobs, Adrian Marc Simon (Oxford, GB) Hector, Jason Roderick (Oxford, GB) Brownlow, Michael James (Oxfordshire, GB) Adachi, Masahiro (Osaka, JP) Skinner, Alison Mary (Mansfield, MA, US) Childs, Mark (Formby Merseyside, GB)