[Journal of Chromatography A] Microfluidic High Performance Liquid Chromatography-Chip Hyphenation t
The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300 nL min−1. The potential of the instrument to eliminate common nanoLC − ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n = 7), and the detection limit was 14 ng mL−1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.
David P. Bishop a, Lucas Blanes a, Alexander B. Wilson a, Thor Wilbanks b, Kevin Killeen b, Rudolf Grimm c, Ross Wenzel a, d, Derek Major e, Mirek Macka f, David Clarke g, Robin Schmid h, Nerida Cole a, i, Philip A. Doble a, a Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia b Agilent Technologies, Santa Clara, CA, USA c University of California Davis, Davis, CA, USA d Pathology NSW, Royal North Shore Hospital, St Leonards, NSW, 2065, Australia e Agnes Banks Equine Clinic, Agnes Banks, NSW, 2753, Australia f School of Physical Sciences and Australian Centre for Research on Separation Science at the University of Tasmania in Hobart, Australia g Teledyne Technologies, Thousand Oaks, CA, USA h Institute of Inorganic and Analytical Chemistry, University of Münster, 48149 Münster, Germany i School of Optometry and Vision Science, University of NSW, Australia Received 3 December 2016, Revised 13 March 2017, Accepted 14 March 2017, Available online 19 March 2017 Show less http://dx.doi.org/10.1016/j.chroma.2017.03.025