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[Conference Article] Ultrafast oscillatory-flow PCR for amplication of urine short tandem repeats


Abstract:

The short tandem repeats (STRs) in human urine which is the most sensitive body fluid can be serviced as biomarkers for the individual identification. PCR microchips are automated, sensitive and rapid. In this report, COMSOL with Finite Element Analysis (FEA) is employed to study the temperature distribution of the base layer to optimize the structure of the microfluidic chip. Meanwhile, the oscillatory PCR microdevice is preliminary tested by DNA samples extracted from urine with the FTA papers and CSF1PO, TPOX, TH01 Multiplex kits. The results show that this method can finish the DNA amplification within 17 minutes for 27 thermal cycling and the annealing as well as denaturing temperature were respectively 60°C and 93 °C.

Conf Proc IEEE Eng Med Biol Soc

Tang H, Dong T.

PMID: 28324958

DOI: 10.1109/EMBC.2016.7591129

Link: https://www.ncbi.nlm.nih.gov/pubmed/28324958

#03242017 #STR #shorttandemrepeat #urine #biomarker #FEA #COMSOL #simulation #oscillatory #PCR

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