[Biosensors and Bioelectronics] Rapid on-chip apoptosis assay on human carcinoma cells based on anne
Despite all the efforts made over years to study the cancer expression and the metastasis event, there is not a clear understanding of its origins and effective treatment. Therefore, more specialized and rapid techniques are required for studying cell behaviour under different drug-based treatments. Here we present a quantum dot signalling-based cell assay carried out in a segmental microfluidic device that allows studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine translocation that occurs in early apoptosis. The developed platform combines the automatic generation of a drug gradient concentration, allowing exposure of cancer cells to different doses, and the immunolabeling of the apoptotic cells using quantum dot reporters. Thereby a complete cell-based assay for efficient drug screening is performed showing a clear correlation between drug dose and amount of cells undergoing apoptosis.
Helena Montóna, b, 1, Mariana Medina-Sáncheza, 1, Joan Antoni Solera, Andrzej Chałupniaka, Carme Noguésb, , Arben Merkoçia, c, , a Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193 Barcelona, Spain b Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Campus UAB-Facultat de Biociències, Bellaterra, 08193 Barcelona, Spain c ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain Received 18 December 2016, Revised 6 March 2017, Accepted 16 March 2017, Available online 18 March 2017